By Rodolfo Paoletti, Dr. David Kritchevsky
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Tokyo) 49, 642. , and Lynen, F. (1961). Biochem. Z. 335, 168. , and Brummer, W. (1962). Angew. Chem. 74, 432. , and Wiegnad, U. (1963). Biochem. Z. 337, 232. Kosow, D. , and Lane, M. D. (1961). Biochem. Biophys. Res. Commun. 5, 191 Kosow, D. , Huang, S. , and Lane, M. D. (1962). /. Biol. Chem. 237, 3633. Lane, M. D. (1966). Personal communication to P. W. Majerus. Lane, M. , and Lynen, F. (1963). Proc. Natl. Acad. Set. S. 49, 379. Lane, M. , Young, D. , and Lynen, F. (1964a). /. Biol. Chem. 239, 2858.
1955). In "Methods in Enzymology" (S. P. Colowick and N. O. ), Vol. I, p. 596. Academic Press, New York. Stern, J. R. (1961). In "The Enzymes" (P. D. Boyer, H. Lardy, and K. ), Vol. 5, Pt. B, p. 511. Academic Press, New York. Fatty Acid Biosynthesis 33 Stumpf, P. , Hawke, J. , and Simoni, R. (1966). In "Biochemistry of Chloroplasts," NATO Advanced Study Inst. ( T. W. Goodwin, ed. ). Academic Press, New York. In press. , and Pogell, B. M. (1966). /. Bid. Chem. 241, 720. Toomey, R. , and Wakil, S.
These experiments indicate that the ACP thioester is the natural substrate for this enzyme and that ACP functions in anaerobic unsaturated fatty acid synthesis. Experiments by Pugh et al. (1966) have confirmed these results and have provided evidence that there are three dehydrases involved in E. colt fatty acid synthesis. One of these is the short-chain dehydrase described by Majerus et al. ( 1965b ) ; another is the ß-y dehydrase; and the third is an α-ß dehydrase that operates at the Cio level to produce saturated long-chain fatty acids.
Advances in lipid research. Volume 5 by Rodolfo Paoletti, Dr. David Kritchevsky